Hidenobu Mizuno, Ph.D.
International Research Center for Medical Sciences, Kumamoto University: Associate Professor
Researchmap↓
http://researchmap.jp/hidenobumizuno/
Curriculum Vitae (March 2018)
(Employment)
2009-2018
Assistant Professor, Division of Neurogenetics. National Institute of Genetics
Research Project: In vivo imaging of activity-dependent development of neuronal circuit in neonatal mouse somatosensory cortex
Synopsis of Research: The aim of my research is to understand how neuronal activity forms neuronal circuits during development. I have studied the development of neuronal circuits in layer 4 somatosensory cortex (barrel cortex) in neonatal mouse. I have used a two-photon microscope to analyze how they develop in living neonates. To visualize individual cortical neurons in vivo, a method to label a sparse population of neurons was developed (Supernova system). Layer 4 neurons
had fluctuating dendrites during formation of the cortical circuit. In NMDA-type glutamate receptor (NMDAR)-deficient neurons, this dendritic motility was enhanced and correct dendrite patterning was disturbed. These results suggest that dendrites of layer 4 neurons search correct synaptic partner during development, and that NMDARs regulate dendritic dynamics to establish fine-tuned circuits (Mizuno et al., Neuron 2014).
2009 (April- August)
Postdoctoral Fellow, Department of Biophysics, Graduate School of Science, Kyoto University (Research Fellow of the Japan Society for the Promotion of Science)
(Education)
2004-2009
Doctoral Course in Science, Department of Biophysics, Graduate School of Science, Kyoto University
Research Project: Roles of neuronal activity in the formation of interhemispheric axon projections in the cerebral cortex
Supervisors: Prof. Tomoo Hirano, Assistant Prof. Yoshiaki Tagawa (currently Lecturer).
Synopsis of Research: I studied the role of neuronal activity in the formation of interhemispheric axon projections in neonatal mouse visual cortex. I used an in utero gene electroporation method to analyze how the spatially organized pattern of interhemispheric axon projections was formed. To explore the role of neuronal activity in their development, an inwardly rectifying potassium channel, Kir2.1, was expressed in interhemispheric projection neurons. Kir2.1 overexpression in interhemispheric projection neurons reduced their firing rate and disturbed the growth of axons and their terminal arborization. These results suggest that interhemispheric connection development is activity-dependent (Mizuno et al., J. Neurosci 2007; Eur. J. Neurosci. 2010).
2000-2004
BSc., Department of Biological Science, School of Science, Nagoya University
Research Project: Molecular mechanisms of laminar restricted projection of hippocampal mossy fibers
Supervisor: Prof. Hajime Fujisawa
(Fellowship)
2008-2009
Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists
(Publications)
Mizuno H, Ikezoe K, Nakazawa S, Sato T, Kitamura K, Iwasato T.
Patchwork-Type Spontaneous Activity in Neonatal Barrel Cortex Layer 4 Transmitted via Thalamocortical Projections.
Cell Rep. 22, 123-135, 2018
Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T.
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.
Sci Rep. 6, 35747, 2016
Iwata R, Matsukawa H, Yasuda K, Mizuno H, Itohara S, Iwasato T.
Developmental RacGAP α2-Chimaerin Signaling Is a Determinant of the Morphological Features of Dendritic Spines in Adulthood.
J Neurosci. 35,13728-13744, 2015
Sakura Mita, Patricia de Monasterio-Schrader, Ursula Funfschilling, Takahiko Kawasaki,
Hidenobu Mizuno, Takuji Iwasato, Klaus-Armin Nave, Hauke B. Werner, Tatsumi Hirata
Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance.
Cerebral Cortex, 25, 4111-4125, 2015
Hidenobu Mizuno, Wenshu Luo, Etsuko Tarusawa, Yoshikazu M. Saito, Takuya Sato, Yumiko Yoshimura, Shigeyoshi Itohara, Takuji Iwasato
NMDAR-Regulated Dynamics of Layer 4 Neuronal Dendrites during Thalamocortical Reorganization in Neonates.
Neuron, 82, 365-379, 2014
Hidenobu Mizuno, Tomoo Hirano, Yoshiaki Tagawa
Pre- and postsynaptic neuron activity supports the axon development of callosal projection neurons during different postnatal periods in the cerebral cortex
The European Journal of Neuroscience, 31, 410-424, 2010
Natsumi Ageta-Ishihara, Sayaka Takemoto-Kimura, Mio Nonaka, Aki Adachi-Morishima, Kanzo Suzuki, Satoshi Kamijo, Hajime Fujii, Tatsuo Mano, Frank Blaeser, Talal Chatila, Hidenobu Mizuno, Tomoo Hirano, Yoshiaki Tagawa, Hiroyuki Okuno, Haruhiko Bito
Control of cortical axon elongation by a GABA-driven Ca2+/calmodulin-dependent protein kinase cascade
The Journal of Neuroscience, 29, 13720-13729, 2009
Yoshiaki Tagawa, Hidenobu Mizuno, Tomoo Hirano
Activity-dependent development of interhemisheric connections in the visual cortex
Reviews in the Neurosciences, 19, 19-28, 2008
Hidenobu Mizuno, Tomoo Hirano, Yoshiaki Tagawa
Evidence for activity-dependent cortical wiring: Formation of interhemispheric connections in neonatal mouse visual cortex requires projection neuron activity
The Journal of Neuroscience, 27, 6760-6770, 2007
Fumikazu Suto, Miu Tsuboi, Haruyuki Kamiya, Hidenobu Mizuno, Yuji Kiyama, Shoji Komai, Masayuki Shimizu, Makoto Sanbo, Takeshi Yagi, Yasushi Hiromi, Alain Chedotal, Kevin J. Mitchell, Toshiya Manabe, Hajime Fujisawa
Interactions between Plexin-A2, Plexin-A4, and Semaphorin 6A Control Lamina-Restricted Projection of Hippocampal Mossy Fibers
Neuron, 53, 535-547, 2007
