Publications （Bold：Selected papers; Highlight: Graduate students)

Banerjee, P., Kubo, F., Nakaoka, H., Ajima, R., Sato, T., Hirata, T., Iwasato, T.
Spontaneous activity in whisker-innervating region of neonatal mouse trigeminal ganglion
Sci Rep. 12(1), 16311. (2022)　　→ Research highlights

Rao, M. S., Mizuno, H., Iwasato, T., Mizuno, H.
Ras GTPase-activating proteins control neuronal circuit development in barrel cortex layer 4
Front Neurosci. 16:901774. (2022)

Sato, H., Hatakeyama, J., Iwasato, T., Araki, K., Yamamoto, N., Shimamura, K.
Thalamocortical axons control the cytoarchitecture of neocortical layers by area-specific supply of VGF.
eLife. 11, e67549 (2022)

Yoshihi, K., Kato, K., Iida, H., Teramoto, M., Kawamura, A., Watanabe, Y., Nunome, M., Nakano, M., Matsuda, Y., Sato, Y., Mizuno, H., Iwasato, T., Ishii, Y., Kondoh, H.
Live imaging of avian epiblast and anterior mesendoderm grafting reveals the complexity of cell dynamics during early brain development.
Development. 149, dev199999. (2022)

Pal, S., Dwivedi, D., Pramanik, T., Godbole, G., Iwasato, T., Jabaudon, D., Bhalla, U.S., Tole, S.
An Early Cortical Progenitor-Specific Mechanism Regulates Thalamocortical Innervation.
J. Neurosci. 41, 6822-6835. (2021)

Nakazawa, S., Iwasato, T.
Spatial organization and transitions of spontaneous neuronal activities in the developing sensory cortex.
Dev Growth Differ. 63, 323-339. (2021)

Mizuno, H., Rao, M., Mizuno, H., Sato, T., Nakazawa, S., Iwasato, T.
NMDA receptor enhances correlation of spontaneous activity in neonatal barrel cortex
J. Neurosci. 41, 1207-1217. (2021)

Iwasato, T.
In Vivo Imaging of Neural Circuit Formation in the Neonatal Mouse Barrel Cortex
Development Growth and Differentiation, 62, 476-486. (2020)

Nakazawa, S., Yoshimura, Y., Takagi, M., Mizuno, H., Iwasato, T.
Developmental Phase Transitions in Spatial Organization of Spontaneous Activity in Postnatal Barrel Cortex Layer 4.
J. Neurosci. 40, 7637-7650. (2020)　→ Research highlights

Iwasato, T., Erzurumlu, R.S.
Development of tactile sensory circuits in the CNS
Current Opinion in Neurobiology, 53:66-75 (2018)

Nakazawa, S., Mizuno, H., Iwasato, T.
Differential dynamics of cortical neuron dendritic trees revealed by long-term in vivo imaging in neonates.
Nature Communications, 9, 3106. (2018)　　→ Press release

Mizuno, H., Nakazawa, S., Iwasato, T.
In vivo 2-photon imaging of cortical neurons in neonatal mice.
J Vis Exp. 140, 58340 (2018)

Mizuno, H., Ikezoe, K., Nakazawa, S., Sato, T., Kitamura, K., Iwasato, T.
Patchwork-Type Spontaneous Activity in Neonatal Barrel Cortex Layer 4 Transmitted via Thalamocortical Projections.
Cell Rep. 22, 123-135. (2018)　　→ Press release

Katori, S., Noguchi-Katori, Y., Okayama, A., Kawamura, Y., Luo, W., Sakimura, K., Hirabayashi, T., Iwasato, T., Yagi, T.
Protocadherin-αC2 is required for diffuse projections of serotonergic axons.
Sci Rep. 7, 15908. (2017)

Katori, S., Noguchi-Katori, Y., Itohara, S., Iwasato, T.
Spinal RacGAP α-chimaerin is required to establish the midline barrier for proper corticospinal axon guidance.
J. Neurosci. 37, 7682-7699. (2017)　　→ Press release

Moreno-Juan, V., Filipchuk, A., Anton-Bolanos, N., Mezzera, C., Gezelius, H., Andres, B., Rodriguez-Malmierca, L., Susin, R., Schaad, O., Iwasato, T., Schuele, R., Rutlin, M., Nelson, S., Ducret, S., Valdeolmillos, M., Rijli, F., Lopez-Bendito, G.
Prenatal thalamic waves regulate cortical area size prior to sensory processing.
Nature Communications, 8, 14172. (2017)

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T.
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.
Sci Rep. 6, 35747. (2016)　　→ Press release

Iwata, R., Iwasato, T.
In vitro Assay for Dendritic Spine Retraction of Hippocampal Neurons with Sparse Labeling.
Bio-Protocol. 6, e1937. (2016)

Shinoda, Y., Ishii, C., Fukazawa, Y., Sadakata, T., Ishii, Y., Sano, Y., Iwasato, T., Itohara, S., Furuichi, T.
CAPS1 stabilizes the state of readily releasable synaptic vesicles to fusion competence at CA3-CA1 synapses in adult hippocampus.
Sci Rep. 6, 31540. (2016)

Iwata, R., Matsukawa, H., Yasuda, K., Mizuno, H., Itohara, S., Iwasato, T.
Developmental RacGAP α2-chimaerin signaling is a determinant of the morphological features of dendritic spines in adulthood
J. Neurosci. 35, 13728-13744 (2015)　　→ Press release

Suzuki, A., Lee, L.J., Hayashi, Y., Muglia, L., Itohara, S., Erzurumlu, R.S., Iwasato, T.
Thalamic adenylyl cyclase 1 is required for barrel formation in the somatosensory cortex.
Neurosci. 290, 518-529. (2015)

Takahashi, A., Lee, R.X., Iwasato, T., Itohara, S., Arima, H., Bettler, B., Miczek, K.A., Koide, T.
Glutamate input in the dorsal raphe nucleus as a determinant of escalated aggression in male mice.
J. Neurosci, 35, 6452-63, (2015)

Mizuno, H., Luo, W., Tarusawa, E., Saito, Y.M., Sato, T., Yoshimura, Y., Itohara, S., Iwasato, T.
NMDAR-Regulated Dynamics of Layer 4 Neuronal Dendrites during Thalamocortical Reorganization in Neonates.　
Neuron 82, 365-379, (2014)　　→ Press release

Iwata, R., Ohi, K., Kobayashi, Y., Masuda, A., Iwama, M., Yasuda, Y., Yamamori, H., Tanaka, M., Hashimoto, R., Itohara, S., Iwasato, T.
RacGAP a2 chimaerin function in development adjusts cognitive ability in adulthood.
Cell Rep. 8, 1257-1264. (2014)　　→ Press release

Arakawa, H., Suzuki, A., Zhao, S., Tsytsarev, V., Lo, F-S. Hayashi, Y., Itohara, S., Iwasato, T., Erzurumlu, R.S.
Thalamic NMDA receptor function is necessary for patterning of the thalamocrotical somatosensory map and for sensorimotor behaviors.
J. Neurosci. 34, 12001-12014. (2014)

Mita, S., de Monasterio-Schrader, P., Funfschilling, U., Kawasaki, T., Mizuno, H., Iwasato, T., Nave, K.A., *Werner, H.B., Hirata, T.
Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance.
Cereb. Cortex, pii: bhu129. [Epub ahead of print] (2014)

Borgius, L., Nishimaru, H., Caldeira, V., Kunugise, Y., Low, P., Reig, R., Itohara, S., Iwasato, T., Kiehn, O.
Spinal Glutamatergic Neurons Defined by EphA4 Signaling Are Essential Components of Normal Locomotor Circuits.
J. Neurosci. 34:3841-53, (2014)

Sadakata, T., Kakegawa, W., Shinoda, Y., Hosono, M., Katoh-Semba, R., Sekine, Y., Sato, Y., Tanaka, M., Iwasato, T., Itohara, S., Furuyama, K., Kawaguchi, Y., Ishizaki, Y., Yuzaki, M., Furuichi, T.
CAPS1 deficiency perturbs dense-core vesicle trafficking and Golgi structure and reduces presynaptic release probability in the mouse brain.
J. Neurosci. 33:17326-34, (2013)

Ogiwara, I., Iwasato, T., Miyamoto, H. Iwata, R., Yamagata, T., Mazaki, E., Yanagawa, Y., Tamamaki, N., Hensch, T.K., Itohara, S., Yamakawa, K.
Nav1.1 haploinsufficiency in excitatory neurons ameliorates seizure-associated sudden death in a mouse model of Dravet syndrome.
Hum Mol Genet. 22:4784-804, (2013)

Hagglund, M., Dougherty, K.J., Borgius, L., Itohara, S., Iwasato, T., Kiehn, O.
Optogenetic dissection reveals multiple rhythmogenic modules underlying locomotion.
Proc Natl Acad Sci U S A. 110:11589-94, (2013)

Yamashita, H., Chen, S., Komagata, S., Hishida, R., Iwasato, T., Itohara, S., Yagi, T., Endo, N., Shibata, M., Shibuki, K.
Restoration of contralateral representation in the mouse somatosensory cortex after crossing nerve transfer.
PLoS One. 2012;7(4):e35676. Epub 2012 Apr 20.

Dhande, O.S., Bhatt, S., Anishchenko, A., Elstrott, J., Iwasato, T., Swindell, E.C., Xu, H.P., Jamrich, M., Itohara, S., Feller, M.B., Crair, M.C.
Role of adenylate cyclase 1 in retinofugal map development.
J. Comp Neurol. 2011 Nov 18. doi: 10.1002/cne.23000. [Epub ahead of print]

Tanabe, Y., Hirano, A., Iwasato, T., Itohara, S., Araki, K., Yamaguchi, T., Ichikawa, T., Kumanishi, T., Aizawa, Y., Takahashi, H., Kakita, A., Nawa, H.
Molecular characterization and gene disruption of a novel zinc-finger protein, HIT-4, expressed in rodent brain.
J. Neurochem. 2010 Feb;112(4):1035-44. Epub 2009 Dec 3.

Singer, P., Yee, B.K., Feldon, J., Iwasato, T., Itohara, S., Grampp, T., Prenosil, G., Benke, D., Mohler, H., Boison, D.
Altered mnemonic functions and resistance to NMDA receptor antagonism by forebrain conditional knockout of glycine transporter 1.
Neuroscience　161, 635-654. (2009)

Takeuchi, S., Yamaki, N., Iwasato, T., Negishi, M., Katoh, H.
Beta 2-chimaerin binds to EphA receptors and regulates cell migration.
FEBS Letters 583, 1237-1242. (2009)

Iwasato, T., Inan, M., Kanki, H., Erzurumlu, R.S., Itohara, S., Crair, M.C.
Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layer IV barrel development.
J. Neurosci. 28, 5931-43. (2008)

Araya, R., Kudo, M., Kawano, M., Ishii, K., Hashikawa, T., Iwasato, T., Itohara, S., Terasaki, T., Oohira, A., Mishina, Y., Yamada, M.
BMP signaling through BMPRIA in astrocytes is essential for proper cerebral angiogenesis and formation of the blood-brain-barrier.
Mol Cell Neurosci. 38, 417-30. (2008)

*Iwasato, T., Katoh, H., Nishimaru, H., Ishikawa, Y., Inoue, H., Saito, Y.M., Ando, R., Iwama, M., Takahashi, R., Negishi, M., Itohara, S.
Rac-GAP α-Chimerin Regulates Motor-Circuit Formation as a Key Mediator of EphrinB3/EphA4 Forward Signaling.
Cell 130, 742-753. (2007)
* The Corresponding Author　　→ Press release

Zeller, A., Crestani, F., Camenisch, I., Iwasato, T., Itohara, S., Fritschy, J.-M., Rudolph, U.
Cortical glutamatergic neurons mediate the motor sedative action of diazepam.
Mol. Pharmacol. 73, 282-91. (2008)

Hirota, Y., Ohshima, T., Kaneko, N., Ikeda, M., Iwasato, T., Kulkarni, A.B., Mikoshiba, K., Okano, H., Sawamoto, K.
Cyclin-dependent kinase 5 is required for control of neuroblast migration in the postnatal subventricular zone.
J. Neurosci. 27, 12829-12838. (2007)

Li, T., Ren, G., Lan, J.Q., Iwasato, T., Itohara, S., Fredholm, B.B., Simon, R.P., Boison, D.
Adenosine kinase, a target for the prediction and prevention of epileptogenesis.
J. Clinic. Invest. 118, 571-82. (2008)

Earnheart, J.C., Schweizer, C., Crestani, F., Iwasato, T., Itohara, S., Mohler, H., Luscher, B.
GABAergic control of adult hippocampal neurogenesis in relation to behavior indicative of chronic trait anxiety and depression states.
J. Neurosci.27, 3845-54. (2007)

Ohshima, T., Hirasawa, M., Tabata, H., Mutoh, T., Adachi, T., Suzuki, H., Saruta, K., Iwasato, T., Itohara, S., Hashimoto, M., Nakajima, K., Ogawa, M., Kulkarni, A.B., Mikoshiba, K.
Cdk5 is required for multipolar-to-bipolar transition during radial neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex.
Development 134, 2273-82. (2007)

Tanaka, K.F., Takebayashi, H., Yamazaki, Y., Ono, K., Naruse, M., Iwasato, T., Itohara, S., Kato, H., Ikenaka, K.
The Murine Model of Alexander Disease: Analyses of GFAP Aggregate Formation and Its Pathological Significance. Glia 55, 617-631 (2007)

Cappello, S., Attardo, A., Wu, X., Iwasato, T., Itohara, S., Wilsch-Brauninger, M., Eilken, H.M., Rieger, M.A., Schroeder, T.T., Huttner, W.B., Brakebusch, C., Gotz, M.
The Rho-GTPase cdc42 regulates neural progenitor fate at the apical surface.
Nature Neurosci. 9, 1099-1107 (2006)

Gu, X., Li, C., Wei, W., Lo, V., Gong, S., Li, S.H., Iwasato, T., Itohara, S., Li, X.J., Mody, I., Heintz, N., Yang, X.W.
Pathological Cell-Cell Interactions Elicited by a Neuropathogenic Form of Mutant Huntingtin Contribute to Cortical Pathogenesis in HD Mice.
Neuron 46, 433-444 (2005)

Lee, L.-J., Iwasato, T., Itohara, S., Erzurumlu, R.
Exuberant Thalamocortical Axon Arborization in Cortex-Specific NMDAR1 Knockout Mice.
J. Comp. Neurol. 485, 280-292 (2005)

Tomioka, R., Okamoto, K., Furuta, T., Fujiyama, F., Yanagawa, Y., Obata, K., Iwasato, T., Kaneko, T., Tamamaki, N.
Demonstration of long-range GABAergic connections distributed throughout the mouse neocortex.
Eur. J. Neurosci. 21, 1587-1600 (2005)

* Iwasato, T., Nomura, R., Ando, R., Ikeda, T., Tanaka, M., *Itohara, S.
Dorsal Telencephalon-Specific Expression of Cre Recombinase in PAC Transgenic Mice.
Genesis 38, 130-138 (2004)
The Corresponding Authors

Yuhki, M., Yamada, M., Kawano, M., Iwasato, T., Itohara, S., Yoshida, H., Ogawa, M., Mishina, Y.
BMP Receptor Signaling is Necessary for Hair Follicle Cycling and Hair Shaft Differentiation in Mice.
Development 131, 1825-1833 (2004)

Inoue, H., Tsukita, K., Iwasato, T., Suzuki, Y., Tomioka, M., Tateno, M., Nagao, M., Misawa, H., Saido, T.C., Miura, M., Itohara, S., Takahashi, R.
The crucial role of caspase-9 in the disease progression of a transgenic ALS mouse model.
EMBO J. 22, 6665-6674 (2003)

Datwani, A., *Iwasato, T., Itohara, S., *Erzurumlu, R.S.,
Lesion-induced thalamocortical axonal plasticity in the S1 cortex is independent of NMDA receptor function in excitatory cortical neurons.
J. Neurosci. 22, 9171-9175 (2002)
* The Corresponding Authors

Datwani, A., Iwasato, T., Itohara, S., Erzurumlu, R.S.
NMDA receptor-dependent pattern transfer from afferents to postsynaptic cells and dendritic differentiation in the barrel cortex.
Mol. Cell Neurosci. 21: 477-92 (2002)

Metzger, F., Repunte-Canonigo, V., Matsushita, S., Akemann, W., Diez-Garcia, J., Ho, C.S., Iwasato, T., Grandes, P., Itohara, S., Joho, R.H., Knopfel, T.
Transgenic mice expressing a pH and Cl- sensing yellow-fluorescent protein under the control of a potassium channel promoter.
Eur. J. Neurosci. 15, 40-50 (2002)

Iwasato, T., Datwani, A., Wolf, A.M., Nishiyama, H., Taguchi, Y., Tonegawa, S., Knopfel, T., Erzurumlu, R.S., Itohara, S.
Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex.
Nature 406, 726-731 (2000)　　→ Press release

Iwasato, T., Erzurumlu, R.S., Chen, D.F., Huerta, P.T., Sasaoka, T., Ulupinar, E., Tonegawa, S.
NMDA receptor dependent refinement of somatosensory maps.
Neuron 19, 1201-1210 (1997)

Iwasato, T., Kanari, Y., Shimizu, A., Honjo, T., Yamagishi, H.
Transforming growth factor-β induced class switch recombination may be modified during the cell proliferation restored by IL-2 stimulation.
Immunol. Lett. 39, 173-178 (1994)

Iwasato, T., Shimizu, T., Kanari, Y., Yamagishi, H.
Molecular characterization of extrachromosomal circular DNAs from an embryonal carcinoma cell line induced to differentiate into neuron-like cells in vivo.
Cell Struct. Funct. 18, 261-266 (1993)

Arakawa, H., Iwasato, T., Hayashida, H., Shimizu, A., Honjo, T., Yamagishi, H.
The complete murine immunoglobulin class switch region of the alpha heavy chain gene – Hierarchic repetitive structure and recombination breakpoints.
J. Biol. Chem. 268, 4651-4655 (1993)

Iwasato, T., Arakawa, H., Shimizu, A., Honjo, T., Yamagishi, H.
Biased distribution of recombination sites within S regions upon immunoglobulin class switch recombination induced by transforming growth factor β and lipopolysaccharide.
J. Exp. Med. 175, 1539-1546 (1992)

Iwasato, T., Yamagishi, H.
Novel excision products of T cell receptor γ gene rearrangements and developmental stage specificity implied by the frequency of nucleotide insertions at signal joints.
Eur. J. Immunol. 22, 101-106. (1992)

Shimizu, T., Iwasato, T., Yamagishi, H.
Deletions of immunoglobulin Cκ region characterized by the circular excision products in mouse splenocytes.
J. Exp. Med. 173, 1065-1072. (1991)

Iwasato, T., Shimizu, A., Honjo, T., Yamagishi, H.
Circular DNA is excised by immunoglobulin class switch recombination.
Cell 62, 143-149 (1990)

Toda, M., Fujimoto, S., Iwasato, T., Takeshita, S., Tezuka, K., Ohbayashi, T., Yamagishi, H.
Structure of extrachromosomal circular DNAs excised from T-cell antigen receptor alpha and delta-chain loci.
J. Mol. Biol. 202, 219-231 (1988)